Peptide dosing & reconstitution calculator
Enter the vial amount, bacteriostatic water, and target amount to get the mg/mL concentration and the exact U-100 syringe units to draw. Handling arithmetic for laboratory research use, not dosing or medical advice.
Reconstitution
Draw for 5-Amino-1MQ
Reference chart at 5 mg/mL
| Target amount | Volume (mL) | U-100 units |
|---|---|---|
| 100 mcg | 0.02 mL | 2 |
| 250 mcg | 0.05 mL | 5 |
| 500 mcg | 0.1 mL | 10 |
| 750 mcg | 0.15 mL | 15 |
| 1000 mcg | 0.2 mL | 20 |
Research-use arithmetic only. This tool converts a vial amount, diluent volume, and a target amount into a concentration and syringe draw for laboratory handling. It is not dosing guidance, a recommendation, or a claim of safety or efficacy, and it does not account for overfill, dead space, or peptide purity. Nothing here is medical advice. “U-100” means 100 units = 1 mL; confirm your syringe graduation before measuring.
How to use the calculator
Four inputs, in order. The result updates as you type.
Select your compound
Pick the research peptide you're reconstituting. On a compound profile the calculator is already set to that peptide.
Enter the vial amount (mg)
This is printed on the vial label — the total amount of lyophilized peptide powder in the vial.
Enter bacteriostatic water (mL)
How much diluent you add. More water means a lower concentration and a larger, easier-to-measure draw.
Enter your target amount
The amount you want per draw, in mcg or mg. The calculator returns the volume and U-100 syringe units.
Worked example
A 10 mg vial reconstituted with 2 mL of bacteriostatic water gives 5 mg/mL, so a 250 mcg target draws to 5 units on a U-100 syringe.
Mixing 10 mg of peptide with 2 mL of BAC water means a full 1 mL syringe (all 100 tick marks) holds 5 mg (5,000 mcg). A 250 mcg target is a fraction of that: 250 mcg ÷ 5,000 mcg/mL = 0.05 mL, which is the 5-unit mark on a U-100 insulin syringe.
Standard bacteriostatic water ratios
Common, even-math mixes by vial size, the concentration each gives, and the U-100 draw for a 250 mcg reference target. These are standard ratios, not usage data — load any vial size into the calculator above to fine-tune the water volume and target for your exact draw.
| Vial size | BAC water | Strength | Units for 250 mcg |
|---|---|---|---|
| 5 mg | 2 mL | 2.5 mg/mL | 10 units |
| 10 mg | 2 mL | 5 mg/mL | 5 units |
| 15 mg | 3 mL | 5 mg/mL | 5 units |
| 20 mg | 2 mL | 10 mg/mL | 2.5 units |
| 30 mg | 3 mL | 10 mg/mL | 2.5 units |
| 40 mg | 4 mL | 10 mg/mL | 2.5 units |
Strength = vial mg ÷ BAC water mL. Units assume a U-100 insulin syringe (1 mL = 100 units).
How to reconstitute a vial
Once the calculator gives you a bacteriostatic water volume, these four handling steps keep the reconstitution careful and consistent.
Sanitize
Wipe the vial stopper with an alcohol swab and let it air-dry for about 10 seconds before proceeding.
Draw the diluent
Draw your calculated volume of bacteriostatic water into a sterile syringe, then insert the needle through the stopper at a slight angle.
Add water to the vial wall
Dispense the water slowly down the inside wall of the vial — never spray it directly onto the powder, which helps preserve peptide integrity.
Mix gently
Swirl or gently roll the vial until the powder fully dissolves. Do not shake — shaking can shear and denature the peptide.
Storage after reconstitution
Once mixed, proper storage helps a research peptide stay stable and lowers the risk of breakdown or contamination.
Refrigerate right away
Store the reconstituted vial at 2–8 °C (36–46 °F) as soon as it's mixed. This is the standard method for everyday research use.
Bacteriostatic water helps
The 0.9% benzyl alcohol in bacteriostatic water slows microbial growth, which makes multi-draw fridge storage more practical than sterile water.
Freeze aliquots for longer
For longer storage, split the solution into small sterile vials and freeze them. Thaw only what you need, and don't refreeze a thawed aliquot.
Avoid these mistakes
Don't repeatedly freeze–thaw one vial, and keep it away from heat and light. Reconstituted peptides are often kept refrigerated for roughly 3–8 weeks.
Which container is the peptide vs. the water?
Research peptide ships as a dry lyophilized powder in a small sealed glass vial. The larger containers in an order are usually the bacteriostatic water diluent.
Small glass vial
An uncommon fill. The small sealed glass vial holds the lyophilized peptide powder, not the diluent.
Standard peptide vial
The 3 mL sealed glass vial is the standard container for lyophilized research peptide across most suppliers.
Higher-fill vial
A larger glass vial used for higher-mg fills, giving more draws per vial for extended research protocols.
Likely BAC water
A 10 mL container in your order is almost certainly your bacteriostatic water, not the peptide.
BAC water diluent
The 15 mL bottle is your bacteriostatic water supply. The peptide is in the small sealed glass vial.
BAC water bottle
A 30 mL bottle is bacteriostatic water. Peptide always ships as dry powder in a compact sealed vial.
Reconstitution FAQ
How do you reconstitute a peptide vial?
You add bacteriostatic water to the lyophilized (freeze-dried) vial. The concentration is the vial amount divided by the water volume: a 10 mg vial reconstituted with 2 mL of bacteriostatic water gives 5 mg/mL (5,000 mcg/mL).
How many units do I draw on an insulin syringe?
On a U-100 syringe, 100 units equal 1 mL. Divide the target amount by the concentration to get the volume in mL, then multiply by 100 for units. At 5 mg/mL, a 250 mcg (0.25 mg) target is 0.05 mL, which is 5 units.
What is bacteriostatic water?
Bacteriostatic water is sterile water with 0.9% benzyl alcohol added to inhibit bacterial growth, which lets a reconstituted vial be used across multiple draws. This calculator works with any diluent volume you enter.
How much bacteriostatic water should I add?
There is no single correct amount — it sets the concentration. More water gives a lower concentration and a larger, easier-to-measure draw; less water gives a smaller draw. Even ratios like a 10 mg vial with 2 mL (5 mg/mL) keep the syringe math simple.
How long does a reconstituted peptide last?
Storage varies by peptide. Reconstituted vials are commonly kept refrigerated at 2–8 °C for roughly 3–8 weeks, and frozen aliquots can last longer. Avoid repeated freeze–thaw cycles.
Does this calculator tell me how much to take?
No. It performs reconstitution and syringe-draw arithmetic for laboratory research handling only. It does not recommend a dose, and nothing here is medical advice. The compounds are for research use, not human or animal consumption.
Women's Peptide Research does not sell peptides or provide dosing or medical advice. Research compounds are for laboratory research and in-vitro use only, not for human or animal consumption. Confirm your syringe graduation before measuring.