For laboratory and educational research use only. Not medical advice.
Women's Peptide Research
Ask Vera
Home  /  Database  /  Irisin
Weight & Metabolicpreclinical

Irisin

FNDC5 fragment
Weight & Metabolic
ASK VERA ABOUT THIS ↗BROWSE THE CATALOG ↗
Category
Weight & Metabolic
Evidence grade
D
PubMed records
2,888
Female-tagged
787
Sex-analysis
19
CAS
—
D
Evidence grade D, Preclinical / animal evidence only across 2888 indexed studies. No human trial data. How grades work →
Female evidence: Extrapolated
Irisin: of 2,888 PubMed records, 787 are tagged as including female subjects and 19 carry a sex-analysis tag.Counts reflect PubMed records as of 2026-07-21. “Female” uses the Female MeSH filter and “sex-analysis” the Sex Factors MeSH filter, automated proxies pending manual study-level review. Methodology →

Overview

Irisin is an exercise-induced myokine, cleaved from the membrane protein FNDC5 and released into circulation, that entered the research conversation as a possible molecular link between physical activity and metabolism. Its identification generated substantial interest because it offered a defined signaling molecule to study rather than a diffuse whole-body effect.

In preclinical models, irisin has been studied for its association with the browning of white adipose tissue, mitochondrial activity, and thermogenic gene expression. Researchers examining energy expenditure, adipose biology, and exercise physiology have used it as a research material for probing how movement translates into metabolic signaling.

For a metabolic research catalog, irisin is notable as a naturally occurring signaling peptide rather than a synthetic analogue, which makes it a useful reference point in myokine and adipose studies. Whether its signaling behaves the same across female physiology remains an open research question; our evidence review tracks how many of the underlying studies included women.

Summary

Irisin is an exercise-induced myokine (cleaved from FNDC5) studied for browning of fat and metabolic and bone effects. It is chiefly investigated as an endogenous signaling molecule and biomarker rather than an established human therapy, and as a research protein it is administered only in cell and animal models; its biology has been examined in women (including bone and PCOS), but not as a compound given to women.

Evidence in women

Menstrual cycle
Unstudied
Circulating irisin has been measured in women, but not systematically across menstrual cycle phases in controlled study.
Pregnancy & lactation
Unstudied
Irisin levels change in pregnancy (observational), but there is no safety data for it as an administered compound.
Hormonal interactions
Unstudied
Irisin biology intersects with estrogen and bone, but no controlled interaction data exist for it as a compound.
Perimenopause & menopause
Unstudied
Irisin is studied observationally in postmenopausal bone loss, but no menopause-specific intervention data exist.
Sex differences
Unstudied
Irisin biology has been examined in women (e.g., PCOS, bone), but it is not an established human therapy and no sex-specific outcomes exist for it as an administered compound.

Mechanism

A myokine released with exercise that is proposed to drive 'browning' of white fat and influence bone and metabolic signaling.

Reported dosing & routes

◐

We don't give dosing or medical advice. The figures below describe what was used in published research, not a recommendation. Decide with your clinician.

Reported range (literature)No established human dose; used as a research protein in preclinical (cell and animal) models only.
Routessubcutaneous, intraperitoneal
Female-specific dosingNot established

Contraindications: insufficient human data to characterize

Side effects

Reported in the research literature. Sex-specific incidence has not been separately analysed in the available records.

insufficient human data to characterize

Irisin reconstitution calculator

Enter this vial's amount, the bacteriostatic water you add, and a target amount to get the concentration and the U-100 syringe units to draw. Handling arithmetic for research use, not a dose recommendation.

Reconstitution

1
2
3

Draw for Irisin

5
units on a U-100 insulin syringe
020406080100
U-100 insulin syringe — 1 mL = 100 units
That's 0.05 mL drawn to the 5-unit mark.
Concentration5 mg/mL (5,000 mcg/mL)
Volume to draw0.05 mL
Doses per vial40

Reference chart at 5 mg/mL

Target amountVolume (mL)U-100 units
100 mcg0.02 mL2
250 mcg0.05 mL5
500 mcg0.1 mL10
750 mcg0.15 mL15
1000 mcg0.2 mL20

Research-use arithmetic only. This tool converts a vial amount, diluent volume, and a target amount into a concentration and syringe draw for laboratory handling. It is not dosing guidance, a recommendation, or a claim of safety or efficacy, and it does not account for overfill, dead space, or peptide purity. Nothing here is medical advice. “U-100” means 100 units = 1 mL; confirm your syringe graduation before measuring. See the reconstitution & storage handbook for handling detail.

Frequently asked questions

Answers drawn only from Irisin's research facts and, where reviewed, its sex-disaggregated evidence. Nothing here is medical advice, dosing, or a claim of safety or efficacy.

What is Irisin?

Irisin, also known as FNDC5 fragment, is a research compound in our weight metabolic category. Irisin is an exercise-induced myokine (cleaved from FNDC5) studied for browning of fat and metabolic and bone effects.

Is Irisin studied in women?

Irisin: of 2,888 PubMed records, 787 are tagged as including female subjects and 19 carry a sex-analysis tag. We grade this extrapolated: women appear in the record, but no study is tagged for a sex-specific analysis, so female-relevant conclusions are inferred from mixed or male data rather than measured in women. See our methodology.

Has Irisin been studied during pregnancy or breastfeeding?

Unstudied. Irisin levels change in pregnancy (observational), but there is no safety data for it as an administered compound. This is not a safety clearance or a recommendation. Vera, our research chat, deliberately hard-stops on pregnancy, breastfeeding, and trying-to-conceive questions rather than answer them.

What evidence grade does Irisin have, and why?

Irisin carries evidence grade D. Preclinical / animal evidence only across 2888 indexed studies. No human trial data.. Grades are derived from the research record, never hand-assigned; see how grades work.

Is there a female-specific dose for Irisin?

No. A female-specific dose for Irisin is not established, and this site does not provide dosing or medical advice. Any figures we show describe what published research used, not a recommendation. Decide anything about use with a qualified clinician.

How is Irisin administered in research?

In the published literature, Irisin is administered via subcutaneous, intraperitoneal. Reported dose ranges on the Dosing tab describe what studies used for laboratory research, not a protocol for people. We don't provide dosing.

What side effects have been reported for Irisin?

Effects reported in the research literature include insufficient human data to characterize. Sex-specific incidence has not been separately analysed in the available records, so these are not broken out for women.

How should Irisin be stored and handled?

Irisin sold for research is supplied as a lyophilized (freeze-dried) powder. Lyophilized peptides are generally kept cold and dry, at roughly -20°C for longer-term storage and 2-8°C short-term, protected from light. Once reconstituted they are typically refrigerated and used within a short window, avoiding repeated freeze-thaw cycles. This is general laboratory handling for research use only, not a directive for human use. Always follow the handling and purity details on the product's Certificate of Analysis.

Where can I buy Irisin for research?

Irisin is not currently listed by our research-use partner, Aurevena Research, but their catalog carries related compounds. Code research15 applies 15% off. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.

Browse the Aurevena catalog
Research-use supplier: Irisin isn't currently listed by our research-use vendor. Their catalog carries related compounds. Code research15 for 15% off, applied automatically at checkout. We may earn a commission when you purchase through this link, at no extra cost to you. Products are for laboratory research use only.
Browse the catalog →

Citations (PMID)

35164383 · 37348598

Related peptides

4X Blend (Methionine / Choline / Carnitine / Dexpanthenol)·
Multi-ingredient blend, its female evidence is reviewed per ingredient, not for the combination.
5-Amino-1MQD
5-Amino-1MQ: of 3 PubMed records, 2 are tagged as including female subjects and 0 carry a sex-analysis tag.
8X Blend (L-Carnitine / L-Arginine / Methionine / Inositol / Choline / B6 / B5 / B12)·
Multi-ingredient blend, its female evidence is reviewed per ingredient, not for the combination.
AdipotideD
Adipotide: of 4 PubMed records, 2 are tagged as including female subjects and 0 carry a sex-analysis tag.

Educational information for laboratory and research use only. Not medical advice, a recommendation, or a claim of safety or efficacy; no personal dosing. “Unstudied” means no qualifying study was found, not that a compound is safe or unsafe.